superhelical dna

In this paper the influence of glucose on the production of supercoiled plasmid DNA in the fermentation process is presented.

本文首先研究了含有不同质量浓度葡萄糖的发酵培养基对超螺旋质粒DNA产量的影响。

The interaction of cupric ions with supercoiled DNA was investigated by agarose gel electrophoresis.

在铜离子浓度一定的条件下 ,随着二者相互作用时间的延长 ,DNA超螺旋结构逐渐开环 ,断裂直至完全消失 。

An anion-exchange high performance liquid chromatographic (HPLC) method was developed to determine the proportion of plasmid DNA in both opened and supercoiled forms.

采用阴离子交换高效液相色谱法监测重组质粒pUDKH的生产过程和该质粒产物中超螺旋pUDKH的比例。

To detect the effect of different proportions of supercoiled plasmid on transfer efficiency and expression level in target cells.

结果表明,用超螺旋比例分别为85。